The Jaakola Lab
Biocenter Oulu and Department of Biochemistry, University of Oulu P.O.Box 3000
FIN-90014 University of Oulu
Finland
E-Mail: veli-pekka.jaakola@oulu.fi
http://www.biochem.oulu.fi/tutkimus/vjaakola/index.html
http://www.biocenter.oulu.fi/
 
We are working with human adenosine receptors and nucleotide transporters. The general aim of the project is to advance current knowledge of the structure-function relationship of adenosine receptors and transporters, and their signalling pathways in the nervous system (http://www.ncbi.nlm.nih.gov/pubmed/20538452). We are looking for a motivated student who is approaching the pro gradu phase of his/her studies. The applicant should have some experience in basic recombinant DNA techniques, protein analyses and cell culture.  
 
The project will mainly include “driving-in” Leishmania tarentolae expression system in our laboratory (http://www.jenabioscience.com/cms/en/1/browse/1007_eukaryotic_expression_system_ lexsy.html). The project can be started in late summer of 2010 or later in the autumn. Details are negotiable. Please send your CV and a short letter explaining why you would be interested in the project to: veli-pekka.jaakola@oulu.fi
 
The methodology for this project will be mainly molecular biological: generation expression cassettes for GPCRs/transporters and various soluble proteins with and without eGFP, cloning, transformation, expression and cultivation optimization etc. in L. tarentolae.  
 
*PCR, fluorescence microscopy, fluorometer, flow-cytometry, radio-ligand-binding, micropurification (His/TALON-magnetics beads), analytical SEC, MS, native/SDS-PAGE and immunostaining.  
 
Methodology papers: (http://www.ncbi.nlm.nih.gov/pubmed/17709746)
(http://www.ncbi.nlm.nih.gov/pubmed/19360018)
http://www.ncbi.nlm.nih.gov/pubmed/17723307
http://www.ncbi.nlm.nih.gov/pubmed/19779853